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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
10/10/2013 |
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Data da última atualização: |
09/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BEVITORI, R.; POPIELARSKA-KONIECZNA, M.; SANTOS, E. M. dos; GROSSI-DE-SÁ, M. F.; PETROFEZA, S. |
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Afiliação: |
ROSANGELA BEVITORI, CNPAF; M. POPIELARSKA-KONIECZNA, JAGIELLONIAN UNIVERSITY, CRACOW-POLAND; E. M. DOS SANTOS, UFG; MARIA FATIMA GROSSI DE SA, CENARGEN; S. PETROFEZA, UFG. |
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Título: |
Morpho-anatomical characterization of mature embryo-derived callus of rice (Oryza sativa L.) suitable for transformation. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Protoplasma, New York, v. 251, n. 3, p. 545-554, May 2014. |
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DOI: |
10.1007/s00709-013-0553-4 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study was to morphoanatomically characterize embryogenic rice calli during early induction of somatic embryogenesis of three Brazilian rice cultivars. Herein, we explored embryogenic units (EUs) from 2-week-old cut proliferated calli to verify whether they were suitable for Agrobacterium tumefasciens-mediated transformation. Histological analysis and scanning electron microscopy (SEM) were used to analyze these types of calli during early rice callogenesis in the cultivars BRS Primavera, BRS Bonança, and BRS Caiapó. The characteristics of the embryogenic cells were preserved in the EUs, which showed a globular, compact structure that contained tightly packed cells and thus rendered the cells suitable for transformation. The EUs of BRS Caiapó also maintained the characteristics of the nonembryogenic callus, such as an elongated morphology and a lack of cellular organization. In general, the observations of the histological sections corresponded with those of the SEM images. The histological analysis suggested that all cultivars used in these experiments have morphogenic potential. The EUs from proliferated 2-week-old cut calli maintained their embryogenic features. The EUs were subjected to Agrobacterium-mediated transformation, which exhibited a regeneration frequency of 58 % for transformed hygromycin-resistant cell lines. These results show that EUs from proliferated 2-week-old cut calli are suitable for plant transformation. |
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Thesagro: |
Arroz; Embriogenese; Morfogenese; Oryza sativa. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/179857/1/Bevitori2014-Article-Morpho-anatomicalCharacterizat.pdf
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Marc: |
LEADER 02199naa a2200229 a 4500
001 1975223
005 2023-03-09
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1007/s00709-013-0553-4$2DOI
100 1 $aBEVITORI, R.
245 $aMorpho-anatomical characterization of mature embryo-derived callus of rice (Oryza sativa L.) suitable for transformation.$h[electronic resource]
260 $c2014
520 $aThe objective of this study was to morphoanatomically characterize embryogenic rice calli during early induction of somatic embryogenesis of three Brazilian rice cultivars. Herein, we explored embryogenic units (EUs) from 2-week-old cut proliferated calli to verify whether they were suitable for Agrobacterium tumefasciens-mediated transformation. Histological analysis and scanning electron microscopy (SEM) were used to analyze these types of calli during early rice callogenesis in the cultivars BRS Primavera, BRS Bonança, and BRS Caiapó. The characteristics of the embryogenic cells were preserved in the EUs, which showed a globular, compact structure that contained tightly packed cells and thus rendered the cells suitable for transformation. The EUs of BRS Caiapó also maintained the characteristics of the nonembryogenic callus, such as an elongated morphology and a lack of cellular organization. In general, the observations of the histological sections corresponded with those of the SEM images. The histological analysis suggested that all cultivars used in these experiments have morphogenic potential. The EUs from proliferated 2-week-old cut calli maintained their embryogenic features. The EUs were subjected to Agrobacterium-mediated transformation, which exhibited a regeneration frequency of 58 % for transformed hygromycin-resistant cell lines. These results show that EUs from proliferated 2-week-old cut calli are suitable for plant transformation.
650 $aArroz
650 $aEmbriogenese
650 $aMorfogenese
650 $aOryza sativa
700 1 $aPOPIELARSKA-KONIECZNA, M.
700 1 $aSANTOS, E. M. dos
700 1 $aGROSSI-DE-SÁ, M. F.
700 1 $aPETROFEZA, S.
773 $tProtoplasma, New York$gv. 251, n. 3, p. 545-554, May 2014.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
03/03/2008 |
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Data da última atualização: |
13/12/2022 |
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Autoria: |
OLIVEIRA, J. S. e; SOUZA SOBRINHO, F. de; LANES, E. C. M. de; ALMEIDA, E. J. D. de. |
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Afiliação: |
Embrapa Gado de Leite. |
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Título: |
Cultivares de milho para silagem: resultados das safras 2003/2004 e 2004/2005. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Juiz de Fora: Embrapa Gado de Leite, 2005. |
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Páginas: |
13 p. |
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Série: |
(Embrapa Gado de Leite. Circular Técnica, 83). |
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Idioma: |
Português |
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Palavras-Chave: |
suplementação volumosa. |
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Thesagro: |
Milho; Nutrição Animal; Ruminante; Silagem. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/65266/1/CT-83-Cultiv-milho-silagem.pdf
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Marc: |
LEADER 00652nam a2200217 a 4500
001 1595697
005 2022-12-13
008 2005 bl uuuu u0uu1 u #d
100 1 $aOLIVEIRA, J. S. e
245 $aCultivares de milho para silagem$bresultados das safras 2003/2004 e 2004/2005.
260 $aJuiz de Fora: Embrapa Gado de Leite$c2005
300 $a13 p.
490 $a(Embrapa Gado de Leite. Circular Técnica, 83).
650 $aMilho
650 $aNutrição Animal
650 $aRuminante
650 $aSilagem
653 $asuplementação volumosa
700 1 $aSOUZA SOBRINHO, F. de
700 1 $aLANES, E. C. M. de
700 1 $aALMEIDA, E. J. D. de
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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